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F (ab') 2 Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Selected Variant

GTR18186234

Size: 1 mg

F (ab') 2 Mouse IgG IgA IgM (H&L) antibody (FITC)

Product Specifications

Product Name Alternative

Goat F (ab') 2 Anti-Mouse IgG IgA IgM Antibody Fluorescein Conjugation, Goat Fab2 Anti-Mouse IgGAM FITC Conjugated Antibody

Reactivity

Mouse

Immunogen

Mouse IgG IgA and IgM whole molecule

Clonality

Polyclonal

Conjugation

FITC

Concentration

1.0 mg/mL

Dilution

IF: 1:500 - 1:2,500

Purity

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Goat IgG F (c), anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

F (ab') 2 Anti-Mouse IgG IgA IgM (H&L) Fluorescein Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity.

Tested Applications

IF, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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