F (ab') 2 Human IgM Fc5u Antibody Biotin Conjugated
Selected Variant
GTR18186209
Size: 500 µg
F (ab') 2 Human IgM Fc5u antibody (Biotin)
Product Specifications
Product Name Alternative
Goat F (ab') 2 Anti-Human IgM Fc5μ Antibody Biotin Conjugation, Goat Fab2 Anti-Human IgM Fc5μ Biotin Conjugated Antibody
Reactivity
Human
Immunogen
Human IgM Fc5μ fragment
Clonality
Polyclonal
Conjugation
Biotin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, FC: 1:200 - 1:1,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgM Fc5μ and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F (c) or other human heavy or light chain proteins.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
F (ab') 2 Anti-Human IgM Fc5μ Biotin Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. F (ab') 2 Anti-Human IgM Fc5μ Biotin Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Tested Applications
ELISA, FC, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG F (ab') 2
Price
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