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F (ab') 2 Human IgM Fc5u Antibody Biotin Conjugated

Selected Variant

GTR18186209

Size: 500 µg

F (ab') 2 Human IgM Fc5u antibody (Biotin)

Product Specifications

Product Name Alternative

Goat F (ab') 2 Anti-Human IgM Fc5μ Antibody Biotin Conjugation, Goat Fab2 Anti-Human IgM Fc5μ Biotin Conjugated Antibody

Reactivity

Human

Immunogen

Human IgM Fc5μ fragment

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, FC: 1:200 - 1:1,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgM Fc5μ and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F (c) or other human heavy or light chain proteins.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

F (ab') 2 Anti-Human IgM Fc5μ Biotin Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. F (ab') 2 Anti-Human IgM Fc5μ Biotin Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

ELISA, FC, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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