Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated
RABBIT IgG (H&L) antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Sheep anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation, sheep anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody
Reactivity
Rabbit
Immunogen
Rabbit IgG whole molecule
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:15,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
Purity
Anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
Anti-Rabbit IgG Alkaline Phosphatase antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry applications. Specific conditions for reactivity should be optimized by the researcher.
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Sheep
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG
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