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Rabbit IgG F (ab') 2 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Rabbit IgG F (ab') 2 antibody (Alkaline Phosphatase)

Product Specifications

Product Name Alternative

Goat Anti-Rabbit IgG F (ab') 2 Antibody alkaline phosphatase Conjugation, Goat Anti-Rabbit IgG Fab2 alkaline phosphatase Conjugated Antibody, Goat Anti-Rabbit IgG Fab2 Fragment alk phos Conjugated Antibody

Reactivity

Rabbit

Immunogen

Rabbit IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

AP

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG, Rabbit IgG F (ab’) 2 and Rabbit Serum. No reaction was observed against Rabbit IgG F (c) or Human Serum Proteins.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

Anti-Rabbit IgG F (ab') 2 Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,100 of the reconstitution concentration is suggested for this product.

Tested Applications

DOT, ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Isotype

IgG

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