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Mouse IgG (H&L) Antibody Peroxidase Conjugated

Mouse IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Chicken anti-Mouse IgG peroxidase Conjugated Antibody, chicken anti-Mouse IgG Antibody HRP Conjugation

Reactivity

Mouse

Immunogen

Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in chicken.

Clonality

Polyclonal

Conjugation

HRP

Concentration

1.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Mouse IgG and Mouse Serum.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Antibody Anti-Mouse IgG (H&L) peroxidase conjugated has been tested by ELISA and western blot and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. T

Tested Applications

ELISA, IHC, WB

Host or Source

Gallus

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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