Mouse IgG2b Antibody Alkaline Phosphatase Conjugated Pre-adsorbed

Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

Store vial at 4° C before opening. DO NOT FREEZE. Conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Anti-Mouse IgG2b Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. Mouse IgG2b alkaline phosphatase conjugated secondary antibodies are ideal for western blot and are available in a variety of formats and conjugate types.

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0