Human IgG (H&L) Antibody Pre-Adsorbed
Human IgG (H&L) antibody
Product Specifications
Product Name Alternative
Human IgG (H&L) Antibody, Rabbit-a-Human IgG (H&L), Human IgG (H&L) Antibody in rabbit, Rabbit anti-human IgG Secondary Antibody.
Reactivity
Human
Immunogen
Anti-Human IgG was produced by repeated immunization with human IgG whole molecule fragment in rabbit.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
2.03 mg/mL
Dilution
ELISA: 1:75,000 - 1:500,000, IHC: 1:1,000 - 1:10,000, WB: 1:10,000 - 1:50,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins. Specificity was confirmed using ELISA minimal cross reaction against Mouse IgG.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Human IgG (H&L) has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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