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Human IgG (H&L) Antibody Pre-Adsorbed

Human IgG (H&L) antibody

Product Specifications

Product Name Alternative

Human IgG (H&L) Antibody, Rabbit-a-Human IgG (H&L), Human IgG (H&L) Antibody in rabbit, Rabbit anti-human IgG Secondary Antibody.

Reactivity

Human

Immunogen

Anti-Human IgG was produced by repeated immunization with human IgG whole molecule fragment in rabbit.

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

2.03 mg/mL

Dilution

ELISA: 1:75,000 - 1:500,000, IHC: 1:1,000 - 1:10,000, WB: 1:10,000 - 1:50,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins. Specificity was confirmed using ELISA minimal cross reaction against Mouse IgG.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Human IgG (H&L) has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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