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Dog IgG F (ab') 2 Antibody Alkaline Phosphatase Conjugated

Dog IgG F (ab') 2 antibody (Alkaline Phosphatase)

Product Specifications

Product Name Alternative

Rabbit anti-dog IgG F (ab') 2 Antibody Alkaline Phosphatase conjugation, rabbit anti-Dog IgG Fab2 fragment Alk Phos conjugated Antibody

Reactivity

Canine

Immunogen

Dog IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

AP

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Dog IgG, Dog IgG F (ab’) 2 and Dog Serum. No reaction was observed against Dog IgG F (c) .

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

Anti-Dog IgG F (ab') 2 Alkaline Phosphatase conjugate has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

DOT, ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Isotype

IgG

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