Bovine IgG (H&L) Antibody Alkaline Phosphatase Conjugated
Bovine IgG (H&L) antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Rabbit anti-Bovine IgG Alkaline Phosphatase Conjugated Antibody, rabbit anti-Bovine IgG Alk phos Conjugated Antibody
Reactivity
Bovine
Immunogen
Bovine IgG whole molecule
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
1 mg/mL
Dilution
ELISA: 1:1,000 - 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Bovine IgG and Bovine Serum.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
Anti-Bovine IgG Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. 0 moles Alkaline Phosphatase (AP) per mole
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000) ; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG
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