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Eif3e Antibody

Eif3S6 Int6 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Eif3s6/Int6 Antibody, rabbit anti-Eif3s6 Antibody, rabbit anti-Int6 Antibody, eIF3e antibody, Eukaryotic translation initiation factor 3 subunit 6 antibody, INT-6 antibody, Viral integration site protein INT 6 antibody, Mammary tumor-associated protein INT-6, MMTV integration site 6, Eukaryotic translation initiation factor 3 subunit E

UniProt

P60229

Reactivity

Human, Monkey, Mouse

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6.

Target

Eif3e

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

0.8 mg/mL

Dilution

ELISA: 1:90,000, WB: 1:1,000

Purity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for mouse eIF3S6/Int6 protein. A BLAST analysis was used to suggest cross-reactivity with most eIF3Se/Int6 isoforms from mouse, human, rat, dog, bovine, and monkey based on 100% homology with the immunizing sequence. Cross-reactivity with EIF3S6/Int6 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to eIF3S6/Int6 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous eIF3S6/Int6.

Tested Applications

ELISA, WB

NCBI Accession Number

45476573

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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