SAE1 Antibody
Sumo Activating Enzyme E1 antibody
Product Specifications
Product Name Alternative
Rabbit anti-Sumo Activating Enzyme E1 Antibody, rabbit anti-SAE1 Antibody, SUMO-activating enzyme subunit 1 antibody, Ubiquitin-like 1-activating enzyme E1A antibody
UniProt
Q9UBE0
Reactivity
Human
Immunogen
Anti-SUMO Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1.
Target
SAE1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
5.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
Purity
This purified antibody is directed against human SUMO Activating Enzyme E1 protein. The product was purified from monospecific antiserum by Protein A chromatography. A BLAST analysis was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%) bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~60 kDa in size corresponding to SAE1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
42559897
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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