Human IgG (H&L) Antibody Peroxidase Conjugated
Human IgG (H&L) antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit anti-Human IgG Antibody peroxidase Conjugation, rabbit anti-Human IgG HRP Conjugated Antibody
Reactivity
Human
Immunogen
Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit.
Clonality
Polyclonal
Conjugation
HRP
Concentration
2.0 mg/mL
Dilution
ELISA: 1:150,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Human IgG (H&L) Peroxidase Conjugated Antibody has been tested by ELISA and Dot Blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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