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F (ab') 2 Rat IgG (H&L) Antibody Fluorescein Conjugated

Selected Variant

GTR18184974

Size: 20 mg

F (ab') 2 Rat IgG (H&L) antibody (FITC)

Product Specifications

Product Name Alternative

Goat F (ab') 2 Anti-Rat IgG fluorescein conjugated Antibody, goat F (ab') 2 Anti-Rat IgG FITC conjugated Antibody, goat Fab2 Anti-Rat IgG antibody fluorescein conjugation

Reactivity

Rat

Immunogen

Rat IgG whole molecule

Clonality

Polyclonal

Conjugation

FITC

Concentration

10.0 mg/mL

Dilution

FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000

Purity

This product is a F (ab’) 2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Goat IgG F (c) or anti-Pepsin.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity.

Tested Applications

FC, FLISA, IF

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Thimerosal. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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