F (ab') 2 Mouse IgG F (ab') 2 Antibody Fluorescein Conjugated
Selected Variant
GTR18184967
Size: 20 mg
F (ab') 2 Mouse IgG F (ab') 2 antibody (FITC)
Product Specifications
Product Name Alternative
Rabbit F (ab') 2 anti-Mouse IgG Antibody fluorescein Conjugated, Rabbit F (ab') 2 anti-Mouse IgG FITC conjugated antibody
Reactivity
Mouse
Immunogen
Mouse IgG F (ab') 2 fragment
Clonality
Polyclonal
Conjugation
FITC
Concentration
10.0 mg/mL
Dilution
FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Purity
This product is a F (ab’) 2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Mouse IgG, Mouse IgG F (ab’) 2 and Mouse Serum. No reaction was observed against Mouse IgG F (c), anti-Rabbit IgG F (c) or anti-Pepsin.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Tested Applications
FC, FLISA, IF
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Thimerosal. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG F (ab') 2
Price
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