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F (ab') 2 Guinea Pig IgG (H&L) Antibody Peroxidase Conjugated

Selected Variant

GTR18184941

Size: 20 mg

F (ab') 2 Guinea Pig IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit F (ab') 2 anti-Guinea Pig IgG Antibody peroxidase Conjugated, Rabbit F (ab') 2 anti-Guinea Pig IgG HRP conjugated antibody

Reactivity

Guinea pig

Immunogen

Guinea Pig IgG whole molecule

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Neuroscience

Concentration

10.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000

Purity

This product is a F (ab’) 2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Rabbit IgG F (c) or anti-Pepsin.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: None. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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