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F (ab') 2 Goat IgG (H&L) Antibody Peroxidase Conjugated

Selected Variant

GTR18184934

Size: 20 mg

F (ab') 2 Goat IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit F (ab') 2 anti-Goat IgG Antibody peroxidase Conjugated, Rabbit F (ab') 2 anti-Goat IgG HRP conjugated antibody

Reactivity

Goat

Immunogen

Goat IgG whole molecule

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Cell Biology

Concentration

10.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000

Purity

This product is a F (ab’) 2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Rabbit IgG F (c) or anti-Pepsin.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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