F (ab') 2 Chicken IgG F (ab') 2 Antibody Fluorescein Conjugated
Selected Variant
GTR18184923
Size: 20 mg
F (ab') 2 Chicken IgG F (ab') 2 antibody (FITC)
Product Specifications
Product Name Alternative
Rabbit F (ab') 2 anti-Chicken IgG F (ab') 2 Antibody Fluorescein Conjugated, Rabbit F (ab') 2 anti-Chicken IgG Fab2 fragment FITC conjugated antibody, Rabbit F (ab') 2 anti-Chicken IgY F (ab') 2 Antibody Fluorescein Conjugated, Rabbit F (ab') 2 anti-Chicken IgY Fab2 fragment FITC conjugated antibody
Reactivity
Gallus
Immunogen
Chicken IgG F (ab') 2 fragment
Clonality
Polyclonal
Conjugation
FITC
Concentration
10.0 mg/mL
Dilution
FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Purity
This product is a F (ab’) 2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Chicken IgG, Chicken IgG F (ab’) 2 and Chicken Serum. No reaction was observed against Chicken IgG F (c), anti-Rabbit IgG F (c) or anti-Pepsin.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
F (ab') 2 Anti-Chicken IgG F (ab') 2 Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity.
Tested Applications
FC, FLISA, IF
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG F (ab') 2
Price
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