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F (ab') 2 Cat IgG (H&L) Antibody Peroxidase Conjugated

F (ab') 2 Cat IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit F (ab') 2 anti-Cat IgG Peroxidase Conjugated Antibody, rabbit F (ab') 2 anti-Cat IgG Antibody HRP Conjugated, rabbit F (ab') 2 anti-Cat IgG Antibody HRP Conjugated

Reactivity

Feline

Immunogen

Cat IgG whole molecule

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Metabolism Research

Concentration

10.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000

Purity

This product is a F (ab’) 2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Cat IgG and Cat Serum. No reaction was observed against anti-Rabbit IgG F (c) or anti-Pepsin.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: None. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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