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Sheep IgG F (ab') 2 Antibody

Sheep IgG F (ab') 2 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Sheep IgG F (ab') 2 fragment Antibody, rabbit anti-Sheep IgG Fab2 fragment Antibody, rabbit anti Sheep IgG Fab2

Reactivity

Sheep

Immunogen

Sheep IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

10.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F (ab’) 2 and Sheep Serum. No reaction was observed against Sheep IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Sheep IgG F (ab’) 2 antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: None. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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