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Rat IgG F (ab') 2 Antibody Peroxidase Conjugated

Rat IgG F (ab') 2 antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit anti-Rat IgG F (ab') 2 Peroxidase Conjugated Antibody, rabbit anti-Rat IgG Fab2 fragment HRP Conjugated Antibody

Reactivity

Rat

Immunogen

Rat IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

HRP

Concentration

10.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000

Purity

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG, Rat IgG F (ab’) 2 and Rat Serum. No reaction was observed against Rat IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Rat IgG F (ab’) 2 antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: None. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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