Rat IgG F (ab') 2 Antibody Peroxidase Conjugated
Rat IgG F (ab') 2 antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit anti-Rat IgG F (ab') 2 Peroxidase Conjugated Antibody, rabbit anti-Rat IgG Fab2 fragment HRP Conjugated Antibody
Reactivity
Rat
Immunogen
Rat IgG F (ab') 2 fragment
Clonality
Polyclonal
Conjugation
HRP
Concentration
10.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG, Rat IgG F (ab’) 2 and Rat Serum. No reaction was observed against Rat IgG F (c) .
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Rat IgG F (ab’) 2 antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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