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Human IgG F (ab') 2 Antibody Fluorescein Conjugated

Human IgG F (ab') 2 antibody (FITC)

Product Specifications

Product Name Alternative

Rabbit anti-Human IgG F (ab') 2 fluorescein Conjugated Antibody, rabbit anti-Human IgG F (ab') 2 FITC Conjugated Antibody, rabbit anti-Human IgG Fab2 fragment Antibody fluorescein Conjugation

Reactivity

Human

Immunogen

Human IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

FITC

Concentration

10 mg/mL

Dilution

FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000

Purity

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Human IgG, Human IgG F (ab’) 2 and Human Serum. No reaction was observed against Human IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Human IgG F (ab') 2 Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

FC, FLISA, IF

Host or Source

Rabbit

Preservative

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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