Human IgG (H&L) Antibody Peroxidase Conjugated
Human IgG (H&L) antibody (Peroxidase)
Product Specifications
Product Name Alternative
Goat anti-Human IgG Peroxidase Conjugated Antibody, goat anti-Human IgG HRP Conjugated Antibody, goat anti-Human IgG Antibody Peroxidase Conjugation
Reactivity
Human
Immunogen
Human IgG whole molecule
Clonality
Polyclonal
Conjugation
HRP
Concentration
10 mg/ml
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Human IgG Fluorescein has been tested by ELISA and is ideal for western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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