Human IgG (H&L) Antibody
Human IgG (H&L) antibody
Product Specifications
Product Name Alternative
Goat anti-human IgG, Anti-Human Ab, Human Secondary Antibody, Goat Anti-Human, Human Antibody in Human
Reactivity
Human
Immunogen
Human IgG whole molecule
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
10 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
Anti-HUMAN IgG (H&L) (GOAT) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by researcher.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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