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Horse IgG F (ab') 2 Antibody

Horse IgG F (ab') 2 antibody

Product Specifications

Product Name Alternative

Goat anti-Horse IgG F (ab') 2 fragment Antibody, goat anti-Horse IgG Fab2 fragment Antibody, goat anti Horse IgG Fab2

Reactivity

Equine

Immunogen

Horse IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

10.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Horse IgG, Horse IgG F (ab’) 2 and Horse Serum. No reaction was observed against Horse IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Horse IgG F (ab') 2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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