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Chicken IgG F (ab') 2 Antibody Fluorescein Conjugated

Chicken IgG F (ab') 2 antibody (FITC)

Product Specifications

Product Name Alternative

Rabbit anti-Chicken IgG F (ab') 2 Antibody Fluorescein Conjugated, rabbit anti-Chicken IgG Fab2 FITC Conjugated Antibody, rabbit anti-Chicken IgY F (ab') 2 Antibody Fluorescein Conjugated, rabbit anti-Chicken IgY Fab2 FITC Conjugated Antibody

Reactivity

Gallus

Immunogen

Chicken IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

FITC

Concentration

10.0 mg/mL

Dilution

FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000

Purity

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Chicken IgG, Chicken IgG F (ab’) 2 and Chicken Serum. No reaction was observed against Chicken IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity.

Tested Applications

FC, FLISA, IF

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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