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Rat IgG2a isotype control Biotin Antibody

Rat IgG2a isotype control Biotin Antibody

Product Specifications

Product Name Alternative

Rat Isotype Control, Rat IgG2a Biotin isotype, RAT IgG2a subclass isotype control

Clone

RG2a

Conjugation

Biotin

Source

Rat

Field of Research

Protein Biochemistry

Concentration

1.0 mg/mL

Dilution

ELISA: User Optimized, FLISA: User Optimized, FC: User Optimized, WB: User Optimized

Purity

RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Rat IgG2a confirmed by Dot Blot.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Notes

For research use only.

Applications Notes

RAT IgG2a isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Tested Applications

ELISA, FC, FLISA, WB

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None

Isotype

IgG2a

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