Rat IgG2a isotype control Fluorescein Antibody
Rat IgG2a isotype control Fluorescein Antibody
Product Specifications
Product Name Alternative
Rat Isotype Control, Rat IgG2a FITC isotype, RAT IgG2a subclass isotype control
Clone
RG2a
Conjugation
FITC
Source
Rat
Field of Research
Protein Biochemistry
Concentration
1.0 mg/mL
Dilution
ELISA: 1:2000 - 1:20,000, FC: 1:1000-1:5000
Purity
RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Rat IgG2a confirmed by Dot Blot.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
Notes
For research use only.
Applications Notes
RAT IgG2a isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
Tested Applications
DOT, ELISA, FC
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None
Isotype
IgG2a
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