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Rat IgG1 isotype control Fluorescein Antibody

Rat IgG1 isotype control Fluorescein Antibody

Product Specifications

Product Name Alternative

Rat Isotype Control, Rat IgG1 FITC isotype, RAT IgG1 subclass isotype control

Clone

RG1

Conjugation

FITC

Source

Rat

Field of Research

Protein Biochemistry

Concentration

1.0

Dilution

ELISA: 1:2000 - 1:20,000, FC: 1:1000-1:5000

Purity

RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rat IgG, and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Rat IgG1 confirmed by Dot Blot.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Notes

For research use only.

Applications Notes

RAT IgG1 isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Tested Applications

ELISA, FC

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None

Isotype

IgG1

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