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Rat IgG1 Lambda isotype control Antibody

Rat IgG1 Lambda isotype control Antibody

Product Specifications

Product Name Alternative

Rat Isotype Control, Rat IgG1 isotype, Rat IgG1 lambda isotype

Clone

RG1L

Conjugation

Unconjugated

Source

Rat

Field of Research

Protein Biochemistry

Concentration

1.0 mg/mL

Dilution

FC: 1:1000-1:5000

Purity

RAT IgG1 Lambda isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG1 and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Light and heavy chain composition has been confirmed by SDS-PAGE.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

RAT IgG1 Lambda isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Tested Applications

FC, SDS-PAGE

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Isotype

IgG1

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