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Mouse IgG2a isotype control Biotin Antibody

Mouse IgG2a isotype control Biotin Antibody

Product Specifications

Product Name Alternative

Biotin conjugated Mouse IgG2a subclass isotype control, Mouse IgG2a isotype control biotin conjugation

Clone

M2A

Conjugation

Biotin

Source

Mouse

Field of Research

Protein Biochemistry

Concentration

1.0 mg/mL

Dilution

FC: 1:1000-1:5000, IF: 1:1000-1:5000

Purity

This product has been prepared from in vitro cell culture by selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Mouse IgG2a was proven by Dot Blot.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Mouse IgG2a isotype control has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Tested Applications

ELISA, FC, IF

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG2a

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