Mouse IgG2a isotype control Biotin Antibody
Mouse IgG2a isotype control Biotin Antibody
Product Specifications
Product Name Alternative
Biotin conjugated Mouse IgG2a subclass isotype control, Mouse IgG2a isotype control biotin conjugation
Clone
M2A
Conjugation
Biotin
Source
Mouse
Field of Research
Protein Biochemistry
Concentration
1.0 mg/mL
Dilution
FC: 1:1000-1:5000, IF: 1:1000-1:5000
Purity
This product has been prepared from in vitro cell culture by selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Mouse IgG2a was proven by Dot Blot.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Mouse IgG2a isotype control has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Tested Applications
ELISA, FC, IF
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2a
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