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Mouse IgG3 Kappa isotype Control Antibody

Mouse IgG3 Kappa isotype Control Antibody

Product Specifications

Product Name Alternative

Mouse IgG3 isotype control, Mouse IgG3 subclass isotype control, mouse IgG3 kappa subclass control

Clone

MG3K

Conjugation

Unconjugated

Source

Mouse

Field of Research

Immunology & Inflammation

Concentration

0.953 mg/mL

Dilution

FC: 1:1000-1:5000

Purity

Mouse IgG3 Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Mouse IgG3 kappa isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Tested Applications

FC, SDS-PAGE

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Isotype

IgG3

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