Mouse IgG2a Kappa isotype Control Antibody

Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Mouse IgG2a kappa isotype control has been tested in SDS-Page, ELISA, and western blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.