HIST1H3A Antibody

Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.

Anti-Histone H3 is directed against the human histone 3 protein. The product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. A BLAST analysis was used to suggest reactivity with human and multiple other eukaryotic. Cross-reactivity with histone-3 from other sources have not been determined.

Store H3 antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Histone H3 antibody has been tested for use in ELISA and western blot. This antibody is suitable for IHC. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should be optimized by the end user.