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Fab Biotin Antibody

Fab antibody

Product Specifications

Product Name Alternative

Goat Fab Anti-Biotin Antibody, Goat Fab Fragment Anti-Biotin Antibody

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:5,000

Purity

Fab fragment of Anti-Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-papain or anti-Goat IgG F (c) .

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Fab Anti-Biotin Antibody has been tested by ELISA, dot blot, and SDS-PAGE and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

DOT, ELISA

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG Fab

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