Fab Biotin Antibody
Fab antibody
Product Specifications
Product Name Alternative
Goat Fab Anti-Biotin Antibody, Goat Fab Fragment Anti-Biotin Antibody
Immunogen
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000
Purity
Fab fragment of Anti-Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-papain or anti-Goat IgG F (c) .
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Fab Anti-Biotin Antibody has been tested by ELISA, dot blot, and SDS-PAGE and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
DOT, ELISA
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG Fab
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