F (Ab') 2 Fluorescein Antibody Alkaline Phosphatase Conjugated
Selected Variant
GTR18184633
Size: 1 mg
F (Ab') 2 antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Goat F (ab') 2 anti-Fluorescein Antibody alkaline phosphatase Conjugation, goat F (ab') 2 anti-Fluorescein alk phos Conjugated Antibody, goat F (ab') 2 anti-FITC Antibody alkaline phosphatase Conjugation
Immunogen
Fluorescein conjugated to Goat IgG
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:38,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin. No reaction was observed against anti-Pepsin or anti-Goat IgG F (c) .
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
F (ab') 2 Anti-Fluorescein Antibody Conjugated to Alkaline Phosphatase has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG F (ab') 2
Price
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