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F (Ab') 2 Biotin Antibody Peroxidase Conjugated

F (Ab') 2 antibody (Peroxidase)

Product Specifications

Product Name Alternative

Goat F (ab') 2 Anti-Biotin Peroxidase Conjugated Antibody, Goat F (ab') 2 Anti-Biotin Antibody Peroxidase Conjugation, Goat Fab2 Anti-Biotin Antibody HRP Conjugation

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Clonality

Polyclonal

Conjugation

HRP

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IHC: 1:100 - 1:500, WB: 1:200 - 1:1,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum and Biotin conjugated IgG. No reaction was observed against anti-Pepsin or anti-Goat IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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