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SingleFlowEx CD30 APC

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

Ber-H8

Applications

FC (QC tested)

Concentration

0.10 mg/mL

Format

APC

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Components

1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

References & Citations

*Aalberse JA, Kapitein B, de Roock S, Klein MR, de Jager W, van der Zee R, Hoekstra MO, van Wijk F, Prakken BJ: Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60) . PLoS One. 2011;6 (9) :e24119., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=aalberse+ja+kapitein+b+de+roock+s , *Falini B, Pileri S, Pizzolo G, Dürkop H, Flenghi L, Stirpe F, Martelli MF, Stein H: CD30 (Ki-1) molecule: a new cytokine receptor of the tumor necrosis factor receptor superfamily as a tool for diagnosis and immunotherapy. Blood. 1995 Jan 1;85 (1) :1-14., URL: http://www.ncbi.nlm.nih.gov/pubmed/7803786 , *Berro AI, Perry GA, Agrawal DK: Increased expression and activation of CD30 induce apoptosis in human blood eosinophils. J Immunol. 2004 Aug 1;173 (3) :2174-83., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=berro+ai+perry+ga , *Franke AC, Jung D, Ellis TM: Characterization of the CD30L binding domain on the human CD30 molecule using anti-CD30 antibodies. Hybridoma. 2000 Feb;19 (1) :43-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=franke+ac+jung+d+ellis+tm , *Matsumoto K, Terakawa M, Miura K, Fukuda S, Nakajima T, Saito H: Extremely rapid and intense induction of apoptosis in human eosinophils by anti-CD30 antibody treatment in vitro. J Immunol. 2004 Feb 15;172 (4) :2186-93., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=matsumoto+k+terakawa+m+miura+k, *Use of this product has not been published., URL:

Other References

*Wright CW, Rumble JM, Duckett CS: CD30 activates both the canonical and alternative NF-kappaB pathways in anaplastic large cell lymphoma cells. J Biol Chem. 2007 Apr;282 (14) :10252-62. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17261581?ordin, *Zeiser R, Nguyen VH, Hou JZ, Beilhack A, Zambricki E, Buess M, Contag CH, Negrin RS: Early CD30 signaling is critical for adoptively transferred CD4+CD25+ regulatory T cells in prevention of acute graft-versus-host disease. Blood. 2007 Mar 1;109 (5) :2225-33., URL: https://www.ncbi.nlm.nih.gov/pubmed/17068147?ordin, *Kusanovic JP, Romero R, Hassan SS, Gotsch F, Edwin S, Chaiworapongsa T, Erez O, Mittal P, Mazaki-Tovi S, Soto E, Than NG, Friel LA, Yoon BH, Espinoza J: Maternal serum soluble CD30 is increased in normal pregnancy, but decreased in preeclampsia and small for gestational age pregnancies. J Matern Fetal Neonatal Med. 2007 Aug 28:1-12, URL: https://www.ncbi.nlm.nih.gov/pubmed/17853188?ordin, *Bekiaris V, Withers D, Glanville SH, McConnell FM, Parnell SM, Kim MY, Gaspal FM, Jenkinson E, Sweet C, Anderson G, Lane PJ: Role of CD30 in B/T segregation in the spleen. J Immunol. 2007 Dec 1;179 (11) :7535-43., URL: https://www.ncbi.nlm.nih.gov/pubmed/18025198?ordin, *Polte T, Behrendt AK, Hansen G: Direct evidence for a critical role of CD30 in the development of allergic asthma. J Allergy Clin Immunol. 2006 Oct;118 (4) :942-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17030250?ordin, *Kennedy MK, Willis CR, Armitage RJ: Deciphering CD30 ligand biology and its role in humoral immunity. Immunology. 2006 Jun;118 (2) :143-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/16771849?ordin, *Fischer M, Harvima IT, Carvalho RF, Möller C, Naukkarinen A, Enblad G, Nilsson G: Mast cell CD30 ligand is upregulated in cutaneous inflammation and mediates degranulation-independent chemokine secretion. J Clin Invest. 2006 Oct;116 (10) :2748-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/16964309?ordin, *Blazar BR, Levy RB, Mak TW, Panoskaltsis-Mortari A, Muta H, Jones M, Roskos M, Serody JS, Yagita H, Podack ER, Taylor PA: CD30/CD30 ligand (CD153) interaction regulates CD4+ T cell-mediated graft-versus-host disease. J Immunol. 2004 Sep 1;173 (5) :2933-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/15322151?ordin

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

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