Cyanine (Sheep) Antibody
Cyanine antibody
Product Specifications
Product Name Alternative
Sheep anti Cyanine Antibody
Immunogen
A mixture of Cy3 and Cy5 conjugated KLH
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.1 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000
Purity
Anti-Cyanine (Sheep) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 2-8° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Cyanine (Sheep) Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Tested Applications
ELISA, WB
Host or Source
Sheep
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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