LAMA1/LAMB1 Antibody (Biotin)
Laminin antibody (Biotin)
Product Specifications
Product Name Alternative
Rabbit anti-Laminin antibody biotin conjugation, biotin conjugated rabbit anti-Laminin antibody, LAMA1 antibody, LAMB1 antibody, Laminin A chain antibody, Laminin alpha 1 antibody, Laminin alpha 1 chain antibody, Laminin B1 chain antibody, Laminin subunit beta 1 antibody
UniProt
P07942
Reactivity
Human
Immunogen
Non-reduced 400 kDa human laminin. Immunization occurred after purity assessment of native protein in non-reducing SDS-PAGE system.
Target
LAMA1/LAMB1
Clonality
Polyclonal
Conjugation
Biotin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:2,000 - 1:10,000
Purity
Anti-Laminin has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent) . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Laminin biotin conjugated antibody is suitable for western blotting, IHC, IP and for ELISA and for use with streptavidin reporter reagents. Researchers should determine optimal titers for applications that are not stated below.
Tested Applications
ELISA, IHC, IP, WB
NCBI Accession Number
AAA59486.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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