COL4A1-COL4A6 Antibody (Biotin)
Collagen IV antibody (Biotin)
Product Specifications
Product Name Alternative
Rabbit anti-Collagen Type IV antibody biotin conjugation, biotin conjugated rabbit anti-Collagen Type IV antibody, Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, Collagen alpha-1 (IV) chain, COL4A1
UniProt
P02462
Reactivity
Bovine, Human
Immunogen
Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.
Target
COL4A1-COL4A6
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Cardiovascular Research
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:2,000 - 1:10,000
Purity
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent) . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Notes
For research use only.
Applications Notes
Anti-Collagen Type IV is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Tested Applications
ELISA, IHC, IP, WB
NCBI Accession Number
NP_001290039.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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