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COL4A1-COL4A6 Antibody (HRP)

Collagen IV antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit anti-Collagen Type IV antibody peroxidase conjugation, HRP conjugated rabbit anti-Collagen Type IV antibody, Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, Collagen alpha-1 (IV) chain, COL4A1

UniProt

P02462

Reactivity

Bovine, Human

Immunogen

Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.

Target

COL4A1-COL4A6

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Cardiovascular Research

Concentration

1.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:2,000 - 1:10,000

Purity

Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent) . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Notes

For research use only.

Applications Notes

Anti-Collagen Type IV Peroxidase Conjugated Antibody has been tested by dot blot and is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

DOT, ELISA, IHC, IP, WB

NCBI Accession Number

NP_001290039.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Isotype

IgG

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