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COL1A1 Antibody (HRP)

COL1A1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-collagen type I antibody peroxidase conjugation, HRP conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1

UniProt

P02452

Reactivity

Bovine, Human, Mouse, Rat

Immunogen

Collagen Type I from human and bovine placenta.

Target

COL1A1

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Musculoskeletal & Connective Tissue Research

Concentration

1.0 mg/mL

Dilution

ELISA: 1:3,000 - 1:6,000, FC: 5μg/mL, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:3,000 - 1:6,000

Purity

This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent) . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Notes

For research use only.

Applications Notes

Anti-COLLAGEN Type I Peroxidase Conjugated Antibody is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

ELISA, FC, IHC, IP, WB

NCBI Accession Number

NP_000079.2

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Thimerosal. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Isotype

IgG

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