Biotin Antibody Peroxidase Conjugated
Biotin antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit anti-Biotin Antibody peroxidase Conjugation, HRP conjugated rabbit anti-Biotin Antibody
Immunogen
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Clonality
Polyclonal
Conjugation
HRP
Concentration
1.1 mg/mL
Dilution
ELISA: 1:10,000-1:80,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, biotinylated bovine serum albumin and Biotinylated IgG.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Biotin Antibody Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), immunoprecipitation and most immunological methods requiring high titer and specificity.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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