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Biotin Antibody Peroxidase Conjugated

Biotin antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit anti-Biotin Antibody peroxidase Conjugation, HRP conjugated rabbit anti-Biotin Antibody

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Clonality

Polyclonal

Conjugation

HRP

Concentration

1.1 mg/mL

Dilution

ELISA: 1:10,000-1:80,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, biotinylated bovine serum albumin and Biotinylated IgG.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Biotin Antibody Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), immunoprecipitation and most immunological methods requiring high titer and specificity.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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