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COL6A1-COL6A3 Antibody (FITC)

Collagen Type VI antibody (FITC)

Product Specifications

Product Name Alternative

Rabbit anti-Collagen Type VI antibody fluorescein conjugation, FITC conjugated rabbit anti-Collagen Type VI antibody, Collagen alpha-1 (VI) chain, Collagen VI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody

UniProt

P12109

Reactivity

Bovine, Human

Immunogen

Collagen Type VI from human and bovine placenta

Target

COL6A1-COL6A3

Clonality

Polyclonal

Conjugation

FITC

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:1,000 - 1:5,000

Purity

Anti-Collagen VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent) . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Notes

For research use only.

Applications Notes

Anti-Collagen Type VI (RABBIT) Fluorescein Conjugated Antibody is suitable for western blot, immunoprecipitation, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

ELISA, IHC, IP, WB

NCBI Accession Number

AAH52575.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Isotype

IgG

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