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Biotin Antibody Fluorescein Conjugated

Biotin antibody (FITC)

Product Specifications

Product Name Alternative

Rabbit anti-Biotin Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Biotin Antibody

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Clonality

Polyclonal

Conjugation

FITC

Concentration

1.0 mg/mL

Dilution

IF: 1:500-1:2,500

Purity

Biotin Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Biotinylated IgG and Biotinylated Bovine Serum Albumin.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Biotin Antibody Fluorescein Conjugated has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

IF, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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