PPARG Antibody

Rabbit anti-PPAR gamma pS273 antibody, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG, PPAR gamma

Anti-PPAR gamma pS273 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human PPARG protein.

Anti-PPAR gamma pS273 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human PPAR gamma at the S273 residue. A BLAST analysis was used to suggest cross-reactivity with rat, mouse, primate, human, dog, bovine, and pig based on 100% sequence homology. Cross-reactivity with PPAR gamma pS273 from other sources has not been determined.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-PPAR antibody has been tested by ELISA and dot blot and is useful for Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~57kDa corresponding to the appropriate cell lysate or extract.