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HTT Antibody

Huntington phospho1181) antibody

Product Specifications

Product Name Alternative

Rabbit anti-Huntington pS1181 antibody, rabbit anti-Huntingtin pS1181 antibody, HD protein, HTT, Huntington Disease Protein, Huntington disease, IT15, LOMARS

UniProt

P42858

Reactivity

Human, Mouse, Rat

Immunogen

Anti-Huntingtin pS1181 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Huntington disease protein.

Target

HTT

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

1.0 mg/ml

Dilution

ELISA: 1: 10,000, WB: 1ug/ml

Purity

Anti-Huntingtin pS1181 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of the S1181 residue. A BLAST analysis was used to suggest cross-reactivity with human, mouse, and rat based on 100% sequence homology. Cross-reactivity with Huntington pS1181 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-HTT antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350kDa corresponding to the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_002102.4

Host or Source

Rabbit

Preservative

Preservative: None. Stabilizer: 50% (v/v) Glycerol with 1 mg/ml Bovine Serum Albumin (rAlbumin) ; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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