BLM Antibody

Anti-BLM pS1296 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Bloom syndrome protein.

Anti-BLM pS1296 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human S1296 residue. A BLAST analysis was used to suggest cross-reactivity with human and mouse based on 100% sequence homology. Cross-reactivity with BLM pS1296 from other sources has not been determined.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-BLM pS1296 antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~159kDa corresponding to the appropriate cell lysate or extract.

Preservative: None. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2